Association of plasma PCSK9 levels and atherosclerosis progression beyond LDL-C
Prospective study finds association between plasma PCSK9 level and carotid plaque formation during 10-year follow-up, in an LDL-independent manner, possibly via regulation by PCSK9 of VLDL-c levels
Association between plasma PCSK9 levels and 10-year progression of carotid atherosclerosis beyond LDL-C: A cohort studyLiterature - Xie W et al., Int J Cardiol 2016
Xie W, Liu J, Wang W, et al.
Int J Cardiol 2016; published online ahead of print
Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes degradation of the LDL receptor (LDLR) and is therefore crucial for LDL-C metabolism [1]. Recent randomised trials showed that the addition of PCSK9 inhibitors to maximal statin therapy significantly reduced LDL-C levels by 60% and consequently reduced cardiovascular events by 50% [2,3].Recent studies indicate that PCSK9 may have roles beyond LDL-C regulation in the progression of atherosclerosis [4-10]. Poirier et al showed that PCKS9 activity also affects the very low-density lipoprotein cholesterol (VLDL-C) metabolism as it contributes to the degradation of its receptor (VLDLR) [11]. Additionally, it was reported that the association of plasma PCSK9 levels with cardiovascular events in coronary heart patients was lost after correction for triglycerides in an observational study [12], and clinical trials found that inhibition of PCSK9 also reduces VLDL-C levels dose-dependently [13,14].
To verify an LDL-independent association of plasma PCSK9 levels with progression of atherosclerosis, carotid plaque formation and total plaque area (TPA) were measured in a Chinese multi-provincial patient cohort (CMCS). In this cohort, 643 participants without carotid plaque and free of CV disease or lipid-lowering medication use completed 10 years of follow-up. Furthermore, the association of PCSK9 with other lipoprotein pathways in the progression of atherosclerosis was examined.
Main results
- PCSK9 levels were significantly positively associated with LDL-P, VLDL-C and triglyceride levels in men and women.
- 61.3% of participants had new carotid plaque formation during follow-up. Relative risk of plaque formation was 1.09 (95%CI: 1.03-1.15, P=0.003) per quartile increase in PCSK9 level.
- TPA enlarged by increasing baseline LDL-C (P<0.001) and PCSK9 (P=0.008) levels.
- TPA linearly increased with baseline VLDL-C levels but only when LDL-C levels were low (lowest tertile P-trend: =0.036, second tertile t not-significant P-trend=0.268).
- A linear relationship was observed between PCSK9 and VLDL-C levels regardless of LDL-C levels.
- The association of TPA with PCSK9 levels in those in the lowest or second LDL-C level tertile (<3.59 mmol/L) disappeared after adjustment for VLDL-C levels (p=0.072).
- The association of TPA with PCSK9 levels was modified by smoking (TPA increase of 15.66 mm2 per 100 ng/mL increase in PCSK9 for smokers, 2.39 mm2 for non-smokers, P-interaction=0.008).
Conclusion
This is the first prospective study that shows the association between baseline plasma PCSK9 levels and the 10-year progression of carotid atherosclerosis measured by new plaque formation and TPA in the general population. Smoking may affect the association of PCSK9 levels with TPA. This study suggests an LDL-independent association of PCSK9 levels with atherosclerosis progression, possibly via regulation by PCSK9 of VLDL-C levels.References
1. Lambert G, Sjouke B, Choque B, et al. The PCSK9 decade. J Lipid Res 2012;53:2515-2524
2. Sabatine MS, Giugliano RP, Wiviott SD, et al. Efficacy and safety of evolocumab in reducing lipids and cardiovascular events, N Engl J Med 2015;372:1500-1509
3. Robinson JG, Farnier M, Krempf M, et al. Efficacy and safety of alirocumab in reducing lipids in cardiovascular events. N Engl J Med 2015;372:1489-1499
4. Lakoski SG, Lagace TA, Cohen JC, et al. Genetic and metabolic determinants of plasma PCSK9 levels. J Clin Endocrinol Metab 2009;94:2537–2543
5. Urban D, Poss J, Bohm M, et al. Targeting the proprotein convertase subtilisin/kexin type 9 for the treatment of dyslipidemia and atherosclerosis. J Am Coll Cardiol 2013;62:1401–1408
6. Cui Q, Ju X, Yang T, et al. Serum PCSK9 is associated with multiple metabolic factors in a large Han Chinese population. Atherosclerosis 2010;213:632–636
7. Cariou B, Le Bras M, Langhi C, et al. Association between plasma PCSK9 and gamma-glutamyl transferase levels in diabetic patients. Atherosclerosis 2010;211:700–702
8. Baass A, Dubuc G, Tremblay M, et al. Plasma PCSK9 is associated with age, sex, and multiple metabolic markers in a population-based sample of children and adolescents. Clin Chem 2009;55:1637–1645
9. Dubuc G, Tremblay M, Pare G, et al. A new method for measurement of total plasma PCSK9: clinical applications. J Lipid Res 2010;51:140–149
10. Li S, Guo YL, Xu RX, et al. Association of plasma PCSK9 levels with white blood cell count and its subsets in patients with stable coronary artery disease. Atherosclerosis 2014;234:441–445
11. Poirier S, Mayer G, Benjannet S, et al. The proprotein convertase PCSK9 induces the degradation of low density lipoprotein receptor (LDLR) and its closest family members VLDLR and ApoER2. J Biol
Chem 2008;283:2363–2372
12. Werner C, Hoffmann MM, Winkler K, et al. Risk prediction with proprotein convertase subtilisin/kexin type 9 (PCSK9) in patients with stable coronary disease on statin treatment. Vasc Pharmacol 2014;62:94–102
13. Koren MJ, Scott R, Kim JB, et al. Efficacy, safety, and tolerability of a monoclonal antibody to proprotein convertase subtilisin/kexin type 9 as monotherapy in patients with hypercholesterolaemia (MENDEL): a randomised, double-blind, placebo-controlled, phase 2 study. Lancet 2012;380:1995–2006
14. Sullivan D, Olsson AG, Scott R, et al. Effect of a monoclonal antibody to PCSK9 on low-density lipoprotein cholesterol levels in statinintolerant patients: the GAUSS randomized trial. J Am Med Assoc 2012;308:2497–2506